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Talat
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«Reply #720 on: June 10, 2005, 02:08:00 PM » |
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Nah !!! we crystallize a protein/ enzyme... study its 3D structure binding site / active crystallogaphically
and then design a ligand...and make a complex and crystallize.....
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lib80aks
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«Reply #721 on: June 10, 2005, 02:11:18 PM » |
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then wut info u get by the crystal structure......................................
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Talat
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«Reply #722 on: June 10, 2005, 02:14:51 PM » |
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About the binding site.........what are the constituting residues ..... the architecture / shape of binding site...on that basis we disgn ligand....
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lib80aks
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«Reply #723 on: June 10, 2005, 02:17:24 PM » |
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ya that i got.....but i mean to say.......wut is its aaplication?  ? |
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Talat
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«Reply #724 on: June 10, 2005, 02:20:36 PM » |
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Ok.... these proteins or enzymes that we study are involved in body in various important functions....in diseased states they cause damage to the body n need to be inhibited...so these ligands can be used as drugs against them...of course ligand >>>>drug is a long journey...........
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lib80aks
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«Reply #725 on: June 10, 2005, 02:22:24 PM » |
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ya good work.............by the way wut type of these enzymes r.....some proteases or wut? ? |
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Talat
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«Reply #726 on: June 10, 2005, 02:24:23 PM » |
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both ..... non-enzymatic proteins also.... and enzymes...also our basic target is Phospholipase A2 that is involved in inflammation n rheumatoid arthritis and other inflammatory conditions...till now no drug is there in the market against this enzyme...
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lib80aks
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«Reply #727 on: June 10, 2005, 02:28:15 PM » |
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but quite a no.of antiinflammatery drugs r there in the market.......may be that will not be for this particular lipases......quite a good work.....
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Talat
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«Reply #728 on: June 10, 2005, 02:30:09 PM » |
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these drugs are specifically against other enzymes wjich are involved in the same pathway...but at later steps...PLA2 initiates the pathway........rate limiting step.....so it will be interesting to have a drug specifically against this enzyme....
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lib80aks
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«Reply #729 on: June 10, 2005, 02:32:02 PM » |
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good goin.............really good work............................................
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Talat
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«Reply #730 on: June 10, 2005, 02:34:48 PM » |
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Thanks!!! .............. my thesis work is on PLA2s..........  |
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lib80aks
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«Reply #731 on: June 10, 2005, 02:36:25 PM » |
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Talat
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«Reply #732 on: June 10, 2005, 02:38:33 PM » |
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i will be submitting my thesis most probably this month buddy !!!!!
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lib80aks
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«Reply #733 on: June 10, 2005, 02:39:46 PM » |
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amazin........so tryin for the post doc.........................
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Talat
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«Reply #734 on: June 10, 2005, 02:42:46 PM » |
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yeah...u know its pretty fast....most of my juniors are older than me in the lab !!!!!! i jumped two standards in childhood ;-)
not now..... abhi i will continue in the same lab....
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on eTI community..
